Acetylcholine-Induced Calcium Movements in Hypotonically Washed Ram Spermatozoa

The lipids of ram spermatozoa.

Micro-cell chamber and washed human spermatozoa.

With the development of the Makler counting chamber, the reproducibility and accuracy of computer-assisted semen analysis and of manual determination of sperm concentration and sperm motility were significantly enhanced beyond what is possible with other methods, such as the hemocytometer, the Horwell chamber, the Petroff-Hausser chamber, and Chartpak slides. The use of Micro-Cell, a disposable...

The Activity of Ram Spermatozoa

Leptin Receptor in Ram Epididymal Spermatozoa

This experiment was designed to investigate leptin receptors (Ob-R and Ob-Rb) mRNA in ram epididymal spermatozoa by RT-PCR. Ten testes were obtained from abattoir and epididymal spermatozoa recovery was performed. To purify spermatozoa, motile sperm were isolated by the swim-up procedure. Total RNA was isolated from epididymal spermatozoa and placental cotyledon, as a positive control, and then...

The role of calcium and Ca2+-ATPase in maintaining motility in ram spermatozoa.

Extracellular calcium at millimolar concentrations inhibits collective motility of ejaculated ram spermatozoa. In untreated cells, or when motility was made dependent upon glycolytic activity, there is very small inhibition, but when motility was made dependent upon mitochondrial respiration there is very high inhibition in motility by increasing extracellular Ca2+ concentration. Quercetin, whi...